Composite

Part:BBa_K5102088:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-10-01)


pU6_gRNA3-ProgRAM_tape1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gRNA 3 encoded under the pU6 promoter with a 30 bp spacer, guiding PspCas13b-ADAR2DD complex to the third AUG in the ProgRAM_tape1, and the PspCas13b stem loop.


Source

Part assembled from the backbone available in lab and oligos from a DNA synthesis provider.

References